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KMID : 0371619990150020155
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Journal of Wonkwang Medical Science
1999 Volume.15 No. 2 p.155 ~ p.160
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Fluorescein Leukocytes Angiography Using the Lymphocytes Selected From the Spleen in Rats in vivo
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Jeong Mi-Ran
Lee Suk-Hee
Hwang Jung-Yong
Yang Yun-Sik
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Abstract
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Background: The study on retinal blood flow has been continuously pursued and recently new methods such as targeted dye delivery, fluorescent vesicle system, laser Doppler velocimetry and acridine orange staining method has been presented. The authors developed a new method, fluorescein leukocyte angiography using a scanning laser ophthalmoscope which include external staining of leukocyte with fluorescein dye and reintroducing this fluorescent leukocyte into the blood flow. However, visualization of the leukocytes was limited since the leukocytes used were from the peripheral blood in a small animal.
Methods: The authors removed leukocytes from the spleen and stained these leukocytes which were reintroduced into the blood flow to observe the retinal and choroidal blood circulation. In four pair of rats, one group was injected with the leukocytes of another rat’s spleen and the other group were injected with the leukocytes of each own’s individual spleen and all underwent angiography.
Results: There were only few lymphocytes visualized in the group of rats which eceived allo-injection of leukocytes and in the other group the lymphocytes were numerous and also had a longer existence.
Conclusions: In small animals like rats the visualization of the lymphocytes used from the spleen was better than the those from the peripheral blood and lymphocytes from one own’s spleen was useful for longer periods of visualization.
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KEYWORD
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fluorescein leukocyte angiography, lymphocytes, spleen, scanning laser ophthalmoscope
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